The separation of rat liver mitochondria into two morphologically different fractions by density-gradient centrifugation

Separation of Hela Cells by Colloidal Silica Density Gradient Centrifugation

Using a colloidal silica density gradient, HeLa cells in mitosis were found to have a density of 1.040-1.046 g/cc, lighter than the remaining interphase cells. The mitotic cells could be harvested and cultured after centrifugation, showing growth synchrony by measurement of a peak in mitotic index 21 hr after establishing the culture. By using Colcemid or vinblastine sulfate, HeLa cells were ar...

Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential

Background: Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa. Also, decreased telomere length in spermatozoa of infertile men is associated with the reduction of fertility potential and embryo quality. Density gradient centrifugation (DGC)...

Rat-Liver Mitochondria and other Subcellular Fractions

1. Rat-liver mitochondria showed a decrease in amino acid production after preparation in 0-25m-sucrose containing EDTA (lnm), but an increase in water content. When EDTA was replaced by Mn2+ (Imm) or succinate (lmM), both amino acid production and water content were lowered, whereas preparation in 0-9% potassium chloride caused an increase in both. 2. Amino acid production by rat-liver homogen...

Tissue fractionation studies. 13. Analysis of mitochondrial fractions from rat liver by density-gradient centrifuging.

Use of dextran-40 gradients for separation of pea cotyledon mitochondria into different fractions.

A crude pea (Pisum sativum L. var. Homesteader) mitochrondrial preparation was divided into two equal parts. One part was layered on a Dextran-40 step gradient, and the other on a sucrose step gradient, and they were centrifuged to obtain different bands of particles. The densities at which the particles banded and the mitochondrial respiratory activities of the particles were determined. Dextr...